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OBJECTIVE To establish quantitative analysis of multi -components by single marker （QAMS） method to simultaneously detect the contents of cinnamic acid ，cinnamaldehyde，plantamajoside，verbascoside，isoacteoside，calceolarioside B ， psoralen，isopsoralen，neobavaisoflavone and bavachin in Gushen dingchuan pill ，and to perform quality evaluation of Gushen dingchuan pill by combining with chemical pattern recognition . METHODS High-performance liquid chromatography was adopted . Using psoralen as internal standard ，the relative correction factors of the other 9 components were established ，and the contents of each component were calculated and compared with those determined by external standard method . Cluster analysis ，principal component analysis and partial least squares discrimination analysis were performed by the results of QAMS method ，and the qualities of 15 batches of Gushen dingchuan pills were evaluated . RESULTS The above 10 components showed a good linear relationship in their respective ranges （r＞0.999 0）. RSDs of precision ，repeatability，stability and recovery tests were all lower than 2.00%. There was no significant difference between QAMS method and external standard method （P＞0.05）. The results of cluster analysis and principal component analysi showed that 15 batches of Gushen dingchuan pills could be clustered into 3 categories. The results of partial least squares discrimination analysis showed that psoralen ，verbascoside，cinnamaldehyde and isopsoralen were the main potential markers affecting the quality of Gushen dingchuan pills . CONCLUSIONS Established QAMS method for quantitative control of multi index components and chemical pattern recognition can be used for the quality evaluation of Gushen dingchuan pills .
ABSTRACT
Objective: To observe the efficacy of Gushen Dingchuan pills combined with routine western medicine in treating stable chronic obstructive pulmonary disease (COPD) patients with syndrome of lung and kidney Qi yin deficiency, and investigate its effect on serum levels of S100 calcium binding protein A8/A9 complex (S100A8/A9), nuclear factor (NF) -κB, tumor necrosis factor (TNF) -α, interleukin (IL) -8 and IL-1β. Method:One hundred and ten stable chronic COPD patients were selected and randomly divided into control group (55 cases) and treatment group (55 cases) by the random number table. Cases of control group received routine therapy by reference to the Guidelines for Diagnosis and Treatment for Chronic Obstructive Pulmonary Disease (2013 Revision). In addition to the therapy of control group, cases of treatment group were orally given Gushen Dingchuan pills(1.5-2.0 g/time, tid). Both groups were treated for 12 weeks. Lung function, scores of symptoms of lung and kidney Qi Yin deficiency, life quality St. George's respiratory questionnaire (SGRQ) score, and the efficacy were compared between both groups. Serum levels of S100A8/A9, NF-κB, TNF-α, IL-8 and IL-1β were detected for two groups. Result:After treatment, FEV1% and FEV1/FVC of treatment group were obviously higher, while score of symptoms of lung and kidney Qi deficiency were lower than control group (Pχ2=4.777, PPκB of treatment group were obviously lower than those of control group (Pα, IL-8, IL-1β of treatment group were remarkably lower than control group after treatment (PConclusion:Gushen Dingchuan pills combined with western medicine can treat stable chronic COPD patients with syndrome of lung and kidney Qi Yin deficiency by improving lung function, alleviating symptoms of lung and kidney Qi deficiency, increasing life quality and efficacy and decreasing levels of S100A8/A9 and NF-κB inflammatory factors.
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Objective To investigate the clinical efficacy of gentle moxibustion plus oral Gushen Dingchuan pills in treating the clinical remission stage of bronchial asthma.Methods Ninety patients with bronchial asthma in the clinical remission stage were randomized to groups A,B and C,30 cases each.group A received moxa-stick gentle moxibustion;group B,oral administration of Gushen Dingchuan pills;group C,moxa-stick gentle moxibustion plus oral administration of Gushen Dingchuan pills.The course of treatment was three months in all the three groups.The TCM symptom score and the Asthma Control Test (ACT) score were recorded and serum immunoglobulin E (IgE) content was measured in the two groups before and after treatment.The clinical therapeutic effects were compared between the groups.Results The total efficacy rate was 76.7％ in group A,80.0％ in group B and 93.3％ in group C.there was a statistically significant difference in the total efficacy rate between group C and group A or B (P＜0.05).There were statistically significant pre-/post-treatment differences in the TCM symptom score,the ACT score and serum IgE content in all the three groups (P＜0.01).There were statistically significant post-treatment differences in the TCM symptom score,the ACT score and serum IgE content between between group C and group A or B (P＜0.01,P＜0.05).Conclusion Gentle moxibustion plus oral Gushen Dingchuan pills can improve immunologic function in patients with bronchial asthma in the clinical remission stage.